Figure 5.

Tau-Cl increased the expression of antioxidant enzymes in the post-MCAO brains. Brain samples for qRT-PCR and immunoblot analysis were prepared from cerebral cortex indicated in Figure 3A at 24 h post-MCAO. (A,B) The expression levels of mRNA of HO-1 (A) and NQO1 (B) were determined by qRT-PCR (n = 3). (C–E) The protein levels of HO-1 and Nrf2 were determined by immunoblotting (C), and blots quantified by using the Image J software are presented as arbitrary density units (D,E) (n = 3). Results are presented as mean ± SEM, *** p < 0.001 vs. sham control, and ^### p < 0.001 vs. PBS-treated MCAO controls by one-way ANOVA with Student–Newman–Keuls test.