Fig. 4.

Phosphorylation of the evolutionarily conserved site in the Ykt6 SNARE domain S174 affects its specificity to its binding partners. (A) Quantitative analysis of MS hits that passed the selection criteria described in main text and Materials and Methods. Spectral counts for common interactors were normalized to WT Ykt6. n = 3, independent MS runs. **P < 0.01, ****P < 0.0001. One-way ANOVA, Tukey’s test. (B) Venn diagram representation of all MS hits that passed the selection criteria described in main text and Materials and Methods. (C) Gene ontology analysis for biological processes from the MS hits in A and B. Bar colors resemble key in A.