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. 2021 Mar 5;9(3):257. doi: 10.3390/biomedicines9030257

Figure 4.

Figure 4

Validation experiments by real-time PCR and specific primers for hsa-miR-205-3p, hsa-let-7i-5p, hsa-miR-1255b, hsa-miR-24-1-5p, hsa-miR-545-5p, and hsa-miR-628-5p in a subset of mild/moderate (Mild/Mod.) BPD samples (n = 5) and severe BPD samples (n = 10). Y axes indicate relative expression values after normalization to hsa-miR-16-1-3p, and a mild/moderate calibration sample using the 2−ΔΔCT method [32,35]. Significant differences were determined by t-test using the GraphPad software (* p < 0.05, ** p < 0.01).