Figure 2.

Neuroprotective activity of EVOO extracts against H₂O₂ in SH-SY5Y cells. Cells were treated with 10 µg/mL of the EVOO extracts for 24 h and then exposed to H₂O₂. (A) Cell viability was measured by MTT assay and (B) LDH was measured as LDH activity in in the culture medium as described in Materials and Methods. Each bar represents means ± SEM of at least three independent experiments. Data were analyzed by one-way ANOVA followed by Bonferroni’s test. * p < 0.05 with respect to CTRL; ° p < 0.05 with respect to H₂O₂; § p < 0.05 with respect to plain.