Table 1.
Sequence of reagents used to purify the microplastic samples according to Löder et al. (2017) [17].
| Step | Reagent | Incubation Temperature and Time |
|---|---|---|
| 1 | Hydrogen peroxide 30% | 60 °C, 1 d |
| 2 | Protease in Tris-HCl buffer (1:5, pH = 9) | 50 °C, 1 d |
| 3 | Lipase in Tris-HCl buffer (1:20, pH = 9) | 40 °C, 1 d |
| 4 | Cellulase in NaOAc buffer (1:4, pH = 5) | 40 C, 3 × 1 d |
| 5 | Hydrogen peroxide 30% | 60 C, 1 d |
| 6 | Chitinase in NaOAc (1:20, pH = 5) | 37 °C, 1 d |
| 7 | Hydrogen peroxide 30% | 60 °C, 1 d |
| 8 | Density separation with ZnCl2 (ρ = 1.6–1.8 g.cm−3) | Room temperature, 1–3 d |