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. 2021 Mar 13;22(6):2915. doi: 10.3390/ijms22062915

Figure 3.

Figure 3

Clotrimazole and fenticonazole effect on ERα levels and cell proliferation. (a,b) Western blotting and relative densitometric analyses of ERα cellular levels in MCF-7 cells treated with different doses of clotrimazole (Clo) (a) and fenticonazole (Fenti) (b) for 72 h. The loading control was done by evaluating vinculin expression in the same filter. The experiment was performed in triplicate. Significant differences with respect to 0 sample are calculate by Student t-test and indicated by * (p-value < 0.05), *** (p-value < 0.001). Western blotting (c) and relative densitometric analyses (c’) of ERα cellular levels in Y537S cells treated with clotrimazole (Clo 10 µM) and fenticonazole (Fenti 10 µM) for 72 h. The experiment was performed in triplicate. Significant differences with respect to 0 samples are calculated by the Student t-test and indicated by **** (p-value < 0.0001). Growth curve analyses in MCF-7 (d,e) or in Y537S (f,g) were performed as indicated in the material and method section for 5 days with the indicated doses (color lines) of clotrimazole (Clo) (d,f) or fenticonazole (Fenti) (e,g). Experiments were done twice in quadruplicate.