Figure 7.

The impact of clotrimazole and fenticonazole on E2-induced DNA synthesis and cell proliferation. (a,a’) Bromodeoxyuridine (BrdU) incorporation assay and (b,b’) cell cycle analysis in MCF-7 cells treated with 17β-estradiol (E2 1 nM–24 h) after 72 h pre-treatment with clotrimazole (Clo 10 µM) and fenticonazole (Fenti 10 µM). The experiments have been performed twice in duplicate. Real-time growth curves in MCF-7 cells treated with clotrimazole (Clo 10 µM) (c) and fenticonazole (Fenti 10 µM) (d) in the absence and the presence of 17β-estradiol (E2 1 nM) and with E2 alone (1 nM) for the indicated time points. The graphs show Clo, Fenti, and E2 effect on the normalized cell index (i.e., cell number), which is detected with the xCelligence DP device and calculated at each time point with respect to the control sample. Each sample was measured in a quadruplicate. For details, please see the material and methods section.