Chromosome spreads of male meiocytes. (A–D) Wild type. (A) Pachytene, (B) Metaphase I with 5 bivalents, (C) Metaphase II, and (D) telophase II. (E–I) zyp1-7. (E) Prophase I, (F) Metaphase I with five bivalents (B), (G) Metaphase II, (H) Telophase II, and (I) Metaphase I with four bivalents and one pair of univalents. (J) Metaphase I in msh5, (K) Metaphase I in msh5 zyp1-3, and (L) Metaphase I in msh5 zyp1-5. (Scale bar, 10 µm.) (M) Quantification of bivalents at metaphase I. Cells were categorized according to the number of pairs of univalents/bivalents. The average number of bivalents per cell and the number of analyzed cells are indicated above the bar. The tests are one-way ANOVA followed by Fisher’s LSD test compared with msh5.