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. 2021 Mar 10;10(3):521. doi: 10.3390/plants10030521

Figure 1.

Figure 1

A visible damage (arrowhead) can be observed on a tomato leaf 24 h after application of a Botrytis cinerea spore suspension drop (circle) (a). B. cinerea hyphae (arrow) visible on the leaf epidermis 24 h after the spore suspension application (b). Staining of tomato leaflets with 2′, 7′-dichlorofluorescein diacetate (DCF-DA) for hydrogen peroxide (H2O2) detection after 30 (c) and 240 min (d) of B. cinerea application. Hydrogen peroxide generation is visible as green fluorescence (c,d). Scale Bars: 1 mm in (a), 0.2 mm in (b), and 500 μm in (c,d).