Table 2.
Anti-proliferative and anti-inflammatory potential of Humulus lupulus, Hypericum perforatum and Curcuma amada.
| Plant Extract | Cell Viability HPK, EC80 |
Anti-Proliferative Effect Psoriasis-Like HPK, EC50 |
Inhibition of IL-6 Pso-Like HPK, EC50 |
Inhibition of IL-8 Pso-Like HPK, EC50 |
|---|---|---|---|---|
| Hop (Humulus lupulus) |
0.22 ± 1.15 µg/ml | 0.36 ± 0.02 µg/ml | 0.28 ± 0.26 µg/ml | 0.34 ± 0.44 µg/ml |
| St John’s wort (Hypericum perforatum) |
0.35 ± 1.08 µg/ml | 0.70 ± 0.03 µg/ml | 0.14 ± 0.12µg/ml | 0.28 ± 0.32 µg/ml |
| Mango ginger (Curcuma amada) |
2.12 ± 1.16 µg/ml | 2.33 ± 0.63 µg/ml | 1.08 ± 0.38 µg/ml | 1.38 ± 0.67 µg/ml |
| Control | ||||
| Dithranol | 0.05 ± 0.02 µg/ml | 0.03 ± 0.001 µg/ml | 0.01 ± 0.01 µg/ml | 0.02 ± 0.01 µg/ml |
Cells were treated with the corresponding extracts in a range from 0.2 to 400 µg/mL. For cell viability tests, HPK were incubated with the extracts for 24 h and the CellTiter-Glo2.0 Assay was performed (n = 3). For measurement of cell proliferation, psoriasis-like HPK were treated for 24 h with the corresponding extracts before the BrdU assay labeling was conducted (n = 6). The IL-6 and IL-8 protein expression level was measured by ELISA (n = 3) in the supernatant of psoriasis-like HPKs after 24 h extract treatment. EC50 (half maximal effective concentration) were calculated with the GraphPadPrism program.