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. 2021 Mar 1;11(3):645. doi: 10.3390/ani11030645

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of forskolin on adipocyte differentiation. (a) Oil-Red O staining of forskolin-treated mesenchymal stem cells after stimulation using an adipogenic induction cocktail for 8 days to promote differentiation. Three experiments (n = 3) were carried out and one representative result is shown. (b) Relative triglyceride content quantified via Oil Red O staining in forskolin-treated mesenchymal stem cells after stimulation using an adipogenic induction cocktail for 8 days. Three experiments (n = 3) were carried out. The bars indicate mean ± SD. ^a–c Means with no common superscript are significantly different (p ≤ 0.05). (c) glycerol levels of culture medium in forskolin-treated mesenchymal stem cells after stimulation using an adipogenic induction cocktail for 8 days. Three experiments (n = 3) were carried out. The bars indicate mean ± SD. ^a,b Means with no common superscript are significantly different (p ≤ 0.05). (d) mRNA expression of glucose transporter 4 (glut4) in mesenchymal stem cells treated with the adipogenic induction cocktail and forskolin for 8 days (n = 3). The bars indicate mean ± SD. ^a,b Means with no common superscript are significantly different (p ≤ 0.05).