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. 2021 Mar 14;11(3):206. doi: 10.3390/jpm11030206

Figure 2.

Figure 2

(A,B) MeWo cell lines were transfected with protein vector for SMO wild-type and SMO mutant (pPM-N-D-C-His) in order to introduce the mutation of interest (CAG vs. CGG); (C) Basal SMO expression in A375 cell lines, used as positive control, and MeWo cell lines evaluated by Western Blot assay and qRT-PCR analysis (p < 0.01); (D) SMO expression was evaluated by Western Blot assay and qRT-PCR analysis (p < 0.001) in MeWo cells parental and in the transfected ones. Control: MeWo parental cell line; Wild-type: MeWo cell line transfected with SMO wild-type vector; MeWo cell lines: transfected with SMO mutant vector. ** p < 0.01, *** p < 0.001.