Figure 5.

(A) Schematic representation of experimental strategy. Mice were injected subcutaneously in the right flank with MeWo cells expressing the SMO wild-type or mutant gene (600,000 cells/mouse), as described in Materials and Methods. After 3 weeks (average tumor size 300 mm³) mice were treated with IMQ (25 mg/kg, every other day, by i.p.) for 3 weeks; (B) 3D volume (mm³) and tumor weight (mg) of SMO wild-type and mutant-gene-amplified MeWo tumor xenografts, treated with vehicle or IMQ i.p. Data are means ± SD of five mice in each group; (C) Representative images of four group of mice enclosed into experimental plan: Group 1: SMO wild-type gene-amplified MeWo cell lines, devised into sub-group 1: vehicle control and sub-group 2: IMQ administrated by i.p.; Group 2: SMO mutant-gene-amplified MeWo cell lines, devised into sub-group 1: vehicle control and sub-group 2: IMQ administrated by i.p.