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. 2021 Mar 14;9(3):598. doi: 10.3390/microorganisms9030598

Table 2.

Characteristics of contigs detected with different sequencing and assembly strategies in five E. coli isolates. Circularized contigs are presented in bold.

17-AB00050 17-AB00090 17-AB00432 17-AB00587 17-AB00639
Number of Contigs (Plasmidal Content Relative to Total Contig Length) Number of Circular Contigs/All Contigs < 10 kb Longest Contig [bp] Number of Contigs (Plasmidal Content Relative to Total Contig Length) Number of Circular Contigs/Contigs < 10 kb Longest Contig [bp] Number of Contigs (Plasmidal Content Relative to Total Contig Length) Number of Circular Contigs/Contigs < 10 kb Longest Contig [bp] Number of Contigs (Plasmidal Content Relative to Total Contig Length) Number of Circular Contigs/Contigs < 10 kb Longest Contig [bp] Number of Contigs (Plasmidal Content Relative to Total Contig Length) Number of Circular Contigs/Contigs < 10 kb Longest Contig [bp]
Unicycler-NextSeq 466 (5.5%) 2/2 341,211 186 (4.3%) 3/3 502,527 159 (1.6%) 0/0 314,433 201 (4.2%) 0/0 485,939 183 (7.6%) 2/2 340,946
Flye-PacBio 13 (4.6%) 4/1 4094,393 6 (4.6%) 6/2 5004,742 2 (2.1%) 2/0 4,736,227 3 (4.4%) 3/0 4,938,765 7 (7.9%) 4/1 3,693,252
Flye-ONT 8 (5.3%) 7/0 5,524,427 5 (4.6%) 5/0 4,970,722 3 (2.7%) 3/0 4,728,917 3 (4.4%) 3/0 4,931,189 7 (8.3%) 7/4 4,756,147
Unicycler-PacBio/NextSeq 27 (4.2%) 6/2 4,341,057 7 (4.6%) 7/3 5,004,751 3 (2.4%) 3/0 4,736,229 3 (4.4%) 3/0 4,938,758 13 (8.1%) 7/2 4,763,387
Unicycler-ONT/NextSeq 22 (4.0%) 6/2 5,533,851 7 (4.6%) 7/3 5,004,751 3 (2.4%) 3/0 4,736,229 3 (4.4%) 3/0 4,938,758 9 (8.2%) 9/2 4,763,387