Figure 6.

PSE and Phy inhibition effect on epithelial to mesenchymal transition (EMT) through decreasing N-cadherin expression in MCF-10A cells. The protein levels of EMT mesenchymal marker N-cadherin were assayed by Western blot analysis using IC₁₀ and IC₅₀ concentrations of Phy (A) and PSE (B). The band intensities of target protein were analysed using the Image Studio Lite software. Quantification is relative to the control and normalised to β-actin expression. Flow cytometry analysis of N-cadherin expression after 24, 48 and 72 h treatment with IC₁₀ Phy (C) and PSE (D). (E) Representative fluorescence microscopy images of N-cadherin expression in MCF-10A cells after IC₁₀ PSE and Phy treatment. The green signal represents corresponding protein staining by Alexa Fluor 488 and the blue signal indicates nuclei staining by DAPI. Original magnification x200. The fluorescence staining of N-cadherin was analysed using ImageJ software. Quantification is relative to the control. Data in graphs are shown as mean ± standard deviation (SD) for three separate experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control; Δ p < 0.05, ΔΔ p < 0.01, ΔΔΔ p < 0.001 compared to TGF-β.