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. 2021 Mar 12;11(3):420. doi: 10.3390/biom11030420

Figure 9.

Figure 9

Analysis of the expression of EMT markers in BJ-5ta cells. (A) BJ-5ta cell lysates from treated or untreated cells were immunoblotted with different antibodies. The band intensity of E-cadherin (B), N-cadherin (C), fibronectin (D), alpha smooth muscle actin (α-SMA) (E), Slug (F), p-Smad2/3 (G) and t_Smad2/3 (H) was quantified using the Image Studio Lite software. Quantification is relative to the control and normalised to β-actin expression (* p < 0.05, ** p < 0.01, *** p < 0.001 compared to control; Δ p < 0.05, ΔΔ p < 0.01, ΔΔΔ p < 0.001 compared to TGF-β).