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. 2021 Mar 12;11(3):420. doi: 10.3390/biom11030420

Table 1.

List of Western blot antibodies.

Primary Antibodies Mr (kDa) Origin Dilution Source
Anti-Fibronectin antibody (ab2413) 285, 262 Rabbit 1:1000 Abcam
N-Cadherin (D4R1H) XP Rabbit mAb 140 Rabbit 1:1000 Cell Signaling Technology, Danvers, Massachusetts, United States
E-Cadherin (4A2) Mouse mAb 135 Mouse 1:1000
α-Smooth Muscle Actin (D4K9N) XP Rabbit mAb 42 Rabbit 1:1000
Slug (C19G7) Rabbit mAb 30 Rabbit 1:1000
Phospho_Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) 52, 60 Rabbit 1:1000
Smad2/3 Antibody 52, 60 Rabbit 1:1000
β-Actin (8H10D10) Mouse mAb 45 Mouse 1:2500
Secondary antibodies
Anti-Rabbit IgG HRP - Goat 1:1000 Cell Signaling Technology, Danvers, Massachusetts, United States
Anti-Mouse IgG HRP - Goat 1:1000