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. 2021 Mar 5;10(3):496. doi: 10.3390/plants10030496

Table 2.

Interaction of the year with the irrigation regime and the BR concentrations with the activity of catalase, peroxidase, superoxide dismutase and polyphenol oxidase enzymes and the content of hydrogen peroxide and proline.

Year Irrigation Regime Brassinolides Concentration CAT
Unit mg Protein−1 Min−1
POD
Unit mg Protein−1 Min−1
SOD
Unit mg Protein−1 Min−1
PPO
μmol Min−1
H2O2
mmol g1 FW
Pro
μmol g1 FW
Year 1 Full watering 0 1.47 o ‡ 4.94 h 1.76 i 0.947 fg 125.03 hi 1.84 o
0.5 3.21 i 4.54 j 1.99 h 0.793 hi 120.3 j 2.89 l
1 2.72 jk 3.84 l 2.23 g 0.667 ij 120.95 j 2.84 l
1.5 1.75 n 3.73 lm 1.83 i 0.603 jk 124.99 hi 2.46 m
Watering until flowering 0 3.63 h 5.79 f 2.92 e 0.86 gh 154.61 c 3.48 jk
0.5 3.9 g 6.44 d 2.87 e 1.54 c 152.61 d 3.85 i
1 4.25 e 6.19 e 2.73 f 1.25 d 150.49 ef 4.01 hi
1.5 4.12 ef 5.88 f 2.7 f 0.993 fg 153.05 cd 3.98 hi
Watering until branching 0 5.48 d 6.18 e 3.4 d 1.14 de 169.03 a 6.67 cd
0.5 5.87 c 7.32 a 4.17 a 2.13 a 168.17 a 7.37 a
1 6.58 b 7.15 b 3.83 b 1.74 b 167.47 a 7.1 ab
1.5 7.34 a 6.86 c 3.6 c 1.57 c 167.83 a 6.47 de
Year 2 Full watering 0 2 m 3.62 mn 1.58 jk 0.28 m 120.55 j 1.78 o
0.5 2.38 l 3.51 n 1.33 l 0.347 m 117.74 k 1.95 o
1 2.41 l 2.87 o 0.93 no 0.417 lm 116.04 l 2.21 mn
1.5 2.35 l 1.88 q 0.89 no 0.273 m 120.6 j 1.84 o
Watering until flowering 0 2.77 jk 4.88 h 1.52 k 0.58 jk 126.64 gh 3.24 k
0.5 2.91 j 4.73 i 1.09 m 0.5 kl 127.21 g 3.74 ij
1 2.78 jk 4.26 k 1 mno 0.41 k−m 124.62 i 4.64 g
1.5 2.7 k 3.79 l 0.88 no 0.29 m 125.93 g−i 4.22 h
Watering until branching 0 3.19 i 5.64 g 1.7 ij 0.49 kl 156.42 b 5.8 f
0.5 3.45 h 4.22 k 1.03 mn 0.68 ij 149.33 f 6.85 bc
1 4.03 g 3.59 n 0.94 no 0.79 hi 151.91 de 7.26 a
1.5 3.89 g 2.57 p 0.69 p 1.03 ef 154.4 c 6.33 e

‡ According to Duncan’s multiple ranges test, values followed by the same letter within the columns are not significantly different at a 5% statistical level. † CAT = catalase; POD = peroxidase; SOD = superoxide dismutase; PPO = polyphenol oxidase; H2O2 = hydrogen peroxide; and Pro = proline.