Table 1.

Techniques utilized to measure de novo protein synthesis in neurites.

Protein metabolic labeling	Technique	Label	Detection	References
	BONCAT/ FUNCAT	Noncanonical aminoacids (azide or alkyne)	Covalent cycloaddition reaction with fluorescently tagged or biotinylated reactive group (alkyne or azide)	[18,19]
	SILAC/ pSILAC	Stable isotope	Mass spectrometry	[20,21]
Puromycilation of nascent peptides	SUnSET/ PUNCH-P	tRNA analogue puromycin; biotinylated puromycin	Puromycin immunodetection; biotin-streptavidin conjugation; mass spectrometry following purification of tagged peptides	[22,23,24,25,26]
Translatomic approach	TRAP/ Ribo-Seq	Epitope-tagged ribosome	Epitope immunoprecipitation followed by mRNA purification and detection by RNA-Seq	[27,28]