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. 2021 Mar 3;10(3):532. doi: 10.3390/cells10030532

Figure 3.

Figure 3

Effect of AZD0156 (ATM inhibitor) on in vitro cyst growth in human ADPKD cells. Human ADPKD cysts derived from WT 9-12 cells were treated with either vehicle [0.1% ethanol (EtOH)] or AZD0156 (0.2, 0.5, and 1 μM) for 18 days. (A) Representative images of human ADPKD cysts treated with AZD0156 on Days 12 (upper panel) and 18 (lower panel) (Images were obtained using 10x objective). (B) Mean Cyst diameter on Days 6, 12, and 18 in the experimental groups. * p < 0.05 compared to vehicle, and # p < 0.05 compared to 0.2 µM AZD0156. (C) Mean percentage cytotoxicity (measured using the LDH assay) and (D) Mean cell proliferation (measured by the BrdU assay) following treatment with either AZD0156 or vehicle. * p < 0.05 compared to vehicle and # p < 0.05 compared to 0.2 µM AZD0156 (color of asterix/hash corresponds to the respective line color). All p-values in figure were determined by one-way ANOVA, followed by post hoc analysis with the Tukey–Kramer HSD test, and data presented as means ± SD (n = 6 per group).