Figure 3.

Cell viability and toxicity of iPSC-derived NPCs. (A) Cell embedding scheme. Neurospheres were freshly chopped to 0.1 mm, mixed with ADA-GEL-X precursor at a density of 10,000 spheres/mL (corresponds to 1 × 10⁷ cells/mL), crosslinked, and maintained in differentiation medium. (B) Viability of neurospheres in the hydrogels measured via resazurin reduction to resorufin. Data shown as relative fluorescence units (RFU). 2D as positive and negative (2D lysis, TritonX-100 treated) controls. (C) Cytotoxicity assessment via LDH release after one day of incubation in differentiation medium, normalized to 2D TritonX-100 treated lysis control (n = 3 with 4 technical replicates; *** p < 0.001 compared to 2D control).