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. 2021 Mar 5;9(3):261. doi: 10.3390/biomedicines9030261

Figure 4.

Figure 4

LIVE/DEAD staining of neurospheres in ADA-X hydrogels. Neurospheres were embedded in the indicated hydrogels and cultivated in differentiation medium. Samples were stained with Calcein-AM (LIVE, green) and Ethidium-homodimer (DEAD, red) and analyzed by light-microscopy in fluorescence and brightfield mode. Cell outgrowth was indicated in ADA-GEL-LAM (white arrows). Scale bar 100 µm.