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List of antibodies.
| Antibody | Dilution | Vendor (cat. nr.) |
|---|---|---|
| Pluripotency markers | ||
| Oct-3/4 | 1:200 | Santa Cruz (sc8628) |
| NANOG | 1:100 | Santa Cruz (sc33759) |
| SSEA4 | 1:100 | Millipore (90231) |
| TRA 1-61 | 1:100 | Millipore (90232) |
| TRA 1-81 | 1:100 | Millipore (90233) |
| Markers of three germ layers | ||
| Vimentin | 1:250 | Abcam (ab92547) |
| α-SMA | 1:200 | Abcam (ab5694) |
| GATA4 | 1:200 | Santa Cruz (sc25310) |
| AFP | 1:200 | Santa Cruz (sc-8108) |
| NFH | 1:200 | Abcam (ab8135) |
| Cardiac specific marker | ||
| TNNT2 | 1:200 | ThermoFisher Scientific (MA5-12960) |
