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Changes in TNF-α levels in plasma and intestinal tissue during MTX induced intestinal damage and exposure to n-3 PUFAs. Enzyme-linked immunosorbent assay (ELISA) was used to determine TNF-α levels. Values are mean ± SEM. CONTR- control, MTX-methotrexate, n-3 PUFAs. * p < 0.05 all groups vs. CONTR rats, † p < 0.05 MTX-n-3 PUFAs vs. MTX rats.
