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Effects of MTX and n-3 PUFAs on crypt cell proliferation (A,B) and apoptosis (A,C). Crypt cell proliferation was assessed using 5-bromodeoxyuridine (5-BrdU). Immunohistochemistry for Caspase-3 was performed for identification of apoptotic cells. Values are mean ± SEM. CONTR- control, MTX-methotrexate, n-3 PUFAs. * p < 0.05 all groups vs. CONTR rats, † p < 0.05 MTX+n-3 PUFAs vs. MTX rats.
