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Grade of intestinal inflammation after administration of MTX and treatment with n-3 PUFAs. Immunohistochemistry with CD 68 antibody was performed to identify macrophages (A,C). The number of intraepithelial lymphocytes (IELs) was assessed by H&E staining and was calculated per 10 villi (A,B). Values are mean ± SEM. CONTR- control, MTX-methotrexate, n-3 PUFAs- n-3 polyunsaturated fatty acids. * p < 0.05 all groups vs. CONTR rats, † p < 0.05 MTX+n-3 PUFAs vs. MTX rats.
