Figure 2.

In vitro validation of predicted peptides as potent stimulators of glucose uptake and Glucose transporter type 4 (GLUT4) translocation. Skeletal muscle cells were stimulated with either insulin or predicted natural peptides at the indicated concentrations for 15 min. (A) The effect on glucose uptake is measured, while in (B), the extent of GLUT4 translocation is assessed (one-way ANOVA analysis with Dunnett’s test; *** p < 0.001; data presented are the mean ± SEM of at least 3 independent replicates).