Figure 6.

BRD4 recruits the AP1 transcription factor complex during RSV-infection. (A) Protein abundance boxplots of AP1 complex members and related proteins. Boxplots represent n = 4 biological replicates per experimental group. Each data point represents the mean of n = 2 technical replicates. ** $P_{adj}<0.01$; **** $P_{adj}<0.0001$. (B) Confirmatory western blot and densitometric quantitation, demonstrating that TLR3-induced inflammation in hSAECs triggers the BRD4/c-JUN interaction in a bromodomain-sensitive manner. (+/−) indicates the presence or absence of the respective compound in a given lane. “IgG HC” refers to the anti-BRD4 antibody heavy chain.