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. 2021 Mar 8;13(3):433. doi: 10.3390/v13030433

Figure 3.

Figure 3

HPV16 E7-induced proliferation of cervical cancer cells depend on PKM2. (A) C33A cells stably expressing HPV16 E7 or empty vector were generated using retroviral vectors. Cell extracts were analyzed with western blot. (B) Cells described in A were seeded in 24-well plates (20,000 cells/well) and counted after 5 days. Results from five independent experiments are shown as mean ± S.E.M. V, vector. * p = 0.005 (two-sided Student’s t-test). (C) C33A-E7 cells were transduced with retrovirus expressing scrambled (SC) shRNA or PKM2 shRNA. Total cell extracts were subject to western blot. (D) Cells described in C were subject to cell counting assay, as described in B. Results from three independent experiments are shown as mean ± S.E.M. * p = 0.01 (two-sided Student’s t-test).