Table 1.
Distribution of 183 resistant genes identified in wastewater isolates from the Beirolas (BEI-DSR/EFL) and Faro (FNw-AFB/EFT) WWTPs (n = 97).
| Gene Name | Gene Product | Antibiotic Resistance | BEI- DSR |
BEI- EFL |
FNw- AFB |
FNw- EFT |
Total Number of Resistance Genes |
|---|---|---|---|---|---|---|---|
| aacA4 | Aminoglycoside 6’-N-acetyltransferase | kanamycin | 1 | 1 | |||
| aadA1 | Streptomycin 3’-adenylyltransferase | streptomycin | 5 | 3 | 1 | 9 | |
| aadA2 | Streptomycin 3’-adenylyltransferase | streptomycin | 6 | 2 | 3 | 11 | |
| aadA5 | Streptomycin 3’-adenylyltransferase | streptomycin | 9 | 7 | 3 | 3 | 22 |
| qnrB-1 | DNA-gyrase and topoisomerase | ciprofloxacin | 2 | 1 | 3 | ||
| qnrB4 | DNA-gyrase and topoisomerase | ciprofloxacin | 1 | 1 | 3 | ||
| qnrB13 | DNA-gyrase and topoisomerase | ciprofloxacin | 1 | 1 | 2 | ||
| aac(6’)-Ib-cr5 | Aminoglycoside 6’-N-acetyltransferase | ciprofloxacin and kanamycin | 19 | 4 | 2 | 1 | 26 |
| dfrA1 | Dihydrofolate reductase | trimethoprim | 3 | 6 | 1 | 10 | |
| dfrA7 | Dihydrofolate reductase | trimethoprim | 3 | 3 | |||
| dfrA12 | Dihydrofolate reductase | trimethoprim | 6 | 2 | 2 | 10 | |
| dfrA15 | Dihydrofolate reductase | trimethoprim | 2 | 2 | |||
| dfrA17 | Dihydrofolate reductase | trimethoprim | 9 | 6 | 4 | 2 | 21 |
| dfrA27 | Dihydrofolate reductase | trimethoprim | 1 | 1 | |||
| arr-3 | Rifampicin ADP-ribosyltransferase | rifamycin | 1 | 1 | 2 | ||
| CTX-M-1 | Class A β-lactamase | cephalosporins | 1 | 1 | |||
| CTX-M-2 | Class A β-lactamase | cephalosporins | 1 | 1 | |||
| CTX-M-14 | Class A β-lactamase | cephalosporins | 2 | 4 | 2 | 8 | |
| CTX-M-15 | Class A β-lactamase | cephalosporins | 22 | 6 | 6 | 3 | 37 |
| CTX-M-27 | Class A β-lactamase | cephalosporins | 1 | 2 | 1 | 4 | |
| CTX-M-32 | Class A β-lactamase | cephalosporins | 1 | 1 | |||
| CTX-M-174 | Class A β-lactamase | cephalosporins | 1 | 1 | |||
| OXY-2 | Class A β-lactamase | cephalosporins | 1 | 1 | |||
| SED-1 | Class A β-lactamase | cephalosporins | 1 | 1 | |||
| KPC-3 | Class A β-lactamase | carbapenem | 1 | 1 | |||
| OXA-2 | Class A β-lactamase | oxacilin | 1 | 1 |
Legend: BEI-DSR: Beirolas-Lisboa influent wastewater; BEI-EFL: Beirolas-Lisboa effluent wastewater; FNw-AFB: Faro Northwest-Algarve influent wastewater; FNw-EFT: Faro Northwest-Algarve effluent wastewater.