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. 2021 Mar 10;11(3):764. doi: 10.3390/ani11030764

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Relative mRNA abundance of paired box transcription factor 7 (PAX7; (A), myogenic factor 5 (MYF5; (B), and myogenic differentiation factor 1 (MYOD; (C) from primary bovine satellite cells cultures following treatment with 10 mM methionine (MET) or 8 mM ornithine (ORN). Cultures were grown to 80% confluency and treated with DMEM/3% horse serum and 10 mM MET or 8 mM ORN. Abundance was measured 0, 2, 4, 8, 12, 24 and 48 h after treatment, as described in the Materials and Methods. The main effects of polyamine precursor, time and polyamine precursor*time in mRNA abundance were analyzed as repeated-measures. Data represent relative mRNA abundance normalized against 18S abundance and are presented as LS mean ± SEM from six separate replicates utilizing BSCs isolated BSCs isolated from at least three different animals. Differences (p < 0.05) between polyamine precursors and the control cultures are indicated by a * at that particular time point.

Relative mRNA abundance of paired box transcription factor 7 (PAX7; (A), myogenic factor 5 (MYF5; (B), and myogenic differentiation factor 1 (MYOD; (C) from primary bovine satellite cells cultures following treatment with 10 mM methionine (MET) or 8 mM ornithine (ORN). Cultures were grown to 80% confluency and treated with DMEM/3% horse serum and 10 mM MET or 8 mM ORN. Abundance was measured 0, 2, 4, 8, 12, 24 and 48 h after treatment, as described in the Materials and Methods. The main effects of polyamine precursor, time and polyamine precursor*time in mRNA abundance were analyzed as repeated-measures. Data represent relative mRNA abundance normalized against 18S abundance and are presented as LS mean ± SEM from six separate replicates utilizing BSCs isolated BSCs isolated from at least three different animals. Differences (p < 0.05) between polyamine precursors and the control cultures are indicated by a * at that particular time point.