Table 1.
Primer and Probe Sequences used in Real-Time PCR.
| Abbreviation | GBA 1 Number | Primers and Probe Sequences, 5′-3′ |
|---|---|---|
| 18S 2 | AF243428 | FP: CCACGCGAGATTGAGCAAT |
| RP: GCAGCCCCGGACATCTAA | ||
| TP: ACAGGTCTGTGATGCC | ||
| PAX7 3 | XM_616352.4 | FP: AGGACGGCGAGAAGAAAGC |
| RP: CCCTTTGTCGCCCAGGAT | ||
| TP: AAGCACAGCATCGAC | ||
| MYF5 4 | NM_174116.1 | FP: AGCCCCACCTCAAGTTGCT |
| RP: GCTGTCAAAACTGCTGCTCTTTC | ||
| TP: CATGCCTGAATGTAAC | ||
| MYOD 5 | NM_001040478.2 | FP: CCGCCTGAGCAAAGTCAAC |
| RP: GGGCAGCCGCTGGTTT | ||
| TP: TGCACGTCTAGCAACC | ||
| MYOG 6 | XM_001790098.1 | FP: CCGCCACGCTGAGAGAGA |
| RP: GGCCTCGAAGGCTTCATTC | ||
| TP: GGCCTCGAAGGCTTCATTC | ||
| ODC 7 | NM_174130 | FP: CTGTACTGATCCTGAGACCTTTG |
| RP: GCTTTACATCCTCTGATCCAGG | ||
| TP: ATCTCTGATGCCCGCTGTGTCTTT | ||
| AMD1 8 | NM_173990 | FP: TGCTGGAGGTTTGGTTCTC |
| RP: TCAAAAGTATGTCCCACTCGG | ||
| TP: TTGGTTTGCGTCGGGTTGCTG |
Forward primer (FP), reverse primer (RP) and TaqMan® probe (TP) sequence along with gene bank accession (GBA) number for the genes analyzed by employing TaqMan® primer probe system of real-time PCR. 1 GenBack Accession Number 2 Ribosomal 18S. 3 Paired box transcription factor 7. 4 Myogenic factor 5. 5 Myogenic differentiation factor 1. 6 Myogenin. 7 Ornithine decarboxylase. 8 S-adenosylmethionine decarboxylase.