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S. aureus PtpB phosphatase activity. Recombinant expressed versions of PtpB, PtpB_T11I, and PtpB_D111A were used to test their phosphatase activity on PNPP in presence (black bars) and absence of DTT (blue bars), respectively. Data are presented as mean + SD of four biological replicates. *,#p < 0.05 (# Mann-Whitney U test between PtpB and its derivatives in presence of DTT; * Mann-Whitney U test between + DTT and –DTT samples).
