Table 2.
Targets | Primers/Probe | Sequences (5′→3′) | References |
---|---|---|---|
lectin | GM1-F | CCA GCT TCG CCG CTT CCT TC | [23] |
GM1-R | GAA GGC AAG CCC ATC TGC AAG CC | ||
GM1-P | FAM-CTT CAC CTT CTA TGC CCC TGA CAC-TAMRA | ||
T-nos | NOS ter 2-5′ | GTC TTG CGA TG ATTA TCA TAT AAT TTC TG | [23] |
NOS ter 2-3′ | CGC TAT ATT TTG TTT TCT ATC GCG T | ||
NOS-Taq | FAM-AGA TGG GTT TTT ATG ATT AGA GTC CCG CAA-TAMRA | ||
T-E9 | T-E9_MF | CGA CAA CGT TCG TCA AGT TC | This study |
T-E9_MR | CCC AAT GCC ATA ATA CTC GAA C | ||
T-E9_NP | FAM-AAT GCA TCA GTT TCA TTG CG-TAMRA | ||
P-RbcS4 | P-RbcS4-F | AAG CAC CAC TCC ACC ATC AC | This study |
P-RbcS4-R | AGG TGT TGA GAC CCT TAT CG | ||
P-RbcS4-P | FAM-ACG TGG CAT TAT TCC AGC GG-TAMRA | ||
pat | Pat-F | CGA TCC ATC TGT TAG GTT GC | [6] |
Pat-R | CCT TGG AGG AGC TGG CAA CT | ||
Pat-P | FAM-ATA CAA GCA TGG TGG ATG GC-TAMRA | This study | |
CV127 | SE-127-f4 | AAC AGA AGT TTC CGT TGA GCT TTA AGA C | [23] |
SE-127-r2 | CAT TCG TAG CTC GGA TCG TGT AC | ||
SE-127-p3 | FAM-TTT GGG GAA GCT GTC CCA TGC CC-TAMRA | ||
DP 305423-1 |
DP305423-f1 | CGT GTT CTC TTT TTG GCT AGC | [23] |
DP305423-r5 | GTG ACC AAT GAA TAC ATA ACA CAA ACT A | ||
DP305423-1p | FAM-TGA CAC AAA TGA TTT TCA TAC AAA AGT CGA GA-TAMRA | ||
DP 356043-5 |
DP356-f1 | GTC GAA TAG GCT AGG TTT ACG AAA AA | [23] |
DP356-r1 | TTT GAT ATT CTT GGA GTA GAC GAG AGT GT | ||
DP356-p | FAM-CTC TAG AGA TCC GTC AAC ATG GTG GAG CAC-TAMRA |