Figure 1.

Yeast two-hybrid (Y2H) analysis of OsKEAP1 and its Kelch repeat domain (OsKelch) with OsABI5. (A) Diagram of OsKEAP1 showing the position of the OsKelch domain. Prediction of the domains was performed at https://smart.embl.de/ (accessed on 5 March 2021). DCD: Development and Cell Death. The green and pink/red boxes represent the coiled coil region and low-complexity region, respectively. (B) Y2H analysis. Yeast cells were transformed with BD-OsABI5 and AD-OsKEAP1 or AD-OsKelch, as indicated. Yeast cells were incubated on DDO (double dropout: SD/-Leu/-Trp) and QDO (quadruple dropout: SD/-Ade/-His/-Leu/-Trp) plates for 5 days.