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Western Blotting analysis of EVs isolated by ultracentrifugation (UC) from untreated (NT), heat-treated (HT) and solvent/detergent (S/D)-treated healthy sera. N = 12. The SDS PAGE of pellets was run in non-reducing conditions (A) and reducing conditions (B). Immunoblotting was performed for TSG101 (C); Alix (D); contaminant Apoliprotein AI (E); tetraspanin CD9 (F) and CD63 (G).
