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Primers used for quantitative PCR for detection of feline infectious coronavirus (FIPV) and house-keeping transcript of 18S ribosomal RNA (18S rRNA) that were used in the study.
| Assay Target | Primers | Specific Product | |
|---|---|---|---|
| Size (Base Pairs) | Tm | ||
| 18S rRNA | 18SrRNA.F: GTA ACC CGT TGA ACC CCA TT 18SrRNA.R: CCA TCC AAT CGG TAG TAG CG |
151 | 83.3 °C |
| FIPV | 26,957F_FCoV: GCA CGT ACT GAC AAT TTG AGT GAA CA 27,054R_FCoV: TCT CCC CAG TTG ACG CGT TGT |
98 | 77.7 °C |
