Figure 2.

Effects of the mutation of the time constants of the inactivation of the Kv2.1, Kv2.2, and Kv1.2 channels. The inactivation time constants for the wild-type and mutant Kv channels were determined by fitting a 5-s depolarization at +20 mV with single exponentials. Red lines show the representative fitting trajectories of the current traces. (A). Representative currents for the Kv2.1 wild-type, W365A, and T370A mutant channels (5-s pulses from −100 mV to 20 mV) (B). Representative currents for the Kv2.2 wild-type, W373A, and T378A mutant channels. (C) Representative currents for the Kv1.2 wild-type, W366A, and S371A mutant channels. (D) Comparison of the time constants of the inactivation of the wild-type and mutant Kv channels, n = 6~12, p > 0.05 (wild type versus mutants).