Figure 6.

Effects of the mutation of the conserved tyrosine residue on the Kv2.1, Kv2.2, and Kv1.2 channels in HEK293 cells. (A) Y376F (Kv2.1) mutant channels present no obvious outward currents. HEK293 cells present no obvious endogenous outward currents. (B,C) Y384F (Kv2.2) and Y377F (Kv1.2) mutant channels present no obvious outward currents. Currents are elicited by a step protocol (holding at −100 mV and depolarizing in 10-mV steps from −100 to +40 mV at 10-s intervals). (D) I-V plots of the outward currents of Kv2.1, Kv2.2, and Kv1.2 wild-type and mutant channels (n = 5~13).