Figure 2.

BMDC that express CR3 and CR4 engage carbohydrate-coated NC to a higher extent when pre-incubated with native serum. BMDC were incubated for 3 h with differentially pretreated (left untreated (w/o), pretreated with native or heat-inactivated (h.i.) serum) carbohydrate-coated types of NC. NC binding to CD11c⁺ DC was assessed by flow cytometry and is given as MFI (mean ± SEM of 3 experiments). Details on the gating strategy are given in Figure S7. Statistical differences versus * w/o serum and ⁺native serum are indicated (one-way ANOVA, Tukey’s test). *^,+ p < 0.05, **^,++ p < 0.01, ***^,+++ p < 0.001.