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. 2021 Mar 13;22(6):2936. doi: 10.3390/ijms22062936

Figure 4.

Figure 4

Analysis of proline in the WT, two BBD1-Ox lines, atbbd1, and atbbd1/atbbd2 plants under drought stress conditions. (A) Free proline contents were measured at designated time points from the leaves of three-week-old soil-grown plants stressed by watering with 20% PEG 6000. (B) The expression of the proline biosynthesis gene P5CS1 was analyzed by qRT-PCR. (C) The expression of the proline catabolic gene PRODH was analyzed with qRT-PCR. The RNA was isolated at designated time points from three-week-old soil-grown plants stressed by watering with 20% PEG 6000. The relative gene expression was calculated and normalized to the reference gene eIF4a1. The normalized mRNA levels at 0 h were set to 1. Data represent the mean ± standard error values of three independent experiments. One-way ANOVA with post-hoc Tukey test was used for the statistical comparison of all genotypes. Different letters indicate significant differences among genotypes at each time point (p < 0.05).