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. 2021 Mar 15;8(3):29. doi: 10.3390/jcdd8030029

Figure 1.

Figure 1

Generation of mouse line with a myocardium specific deletion of Rac1 (Rac1Nkx2.5). A schema of the floxed Rac1 allele and Nkx2.5-Cre mediated generation of the Rac1 null allele (A). Rac1Nkx2.5 mice were generated by crossing Nkx2.5-Cre transgenic mice with Rac1f/f mice for 2 generations with the expected genotype frequencies indicated in brackets (B). Rac1 mRNA expression was significantly reduced in E12.5 Rac1Nkx2.5 ventricular myocardium compared to Rac1f/f littermates (C). Western blot analysis of E12.5 ventricular myocardium showed a significant decrease in Rac1 protein levels in Rac1Nkx2.5 hearts compared to controls (D). * p < 0.05, *** p < 0.001 by unpaired Student’s t-test.

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