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. 2021 Mar 15;8(3):29. doi: 10.3390/jcdd8030029

Figure 2.

Figure 2

Lineage tracing with Nkx2.5-Cre;mT/mG mice. GFP immunostaining of E12.5 Nkx2.5-Cre;mT/mG paraffin heart sections showed Cre recombinase activity in the atrial and ventricular myocardium (A), OFT (B) and aortic valve leaflets (C). Fluorescence imaging of cryosections of E18.5 Nkx2.5-Cre;mT/mG hearts showed that the epicardium (arrows) and endothelial cells (arrowhead) remain RFP+ in Nkx2.5-Cre;mT/mG hearts (D–F). RV, right ventricle; LV, left ventricle; PA, pulmonary artery; Ao, aorta; epi, epicardium. Scale bars: 250 µm (A–C), 10 µm (D–F).