Figure 6.
In vivo effect of LMM6 on treatment of mice with systemic candidiasis by C. albicans. The mice were infected with reference strain (5 × 105 yeast cells) and treated twice a day for 5 days via intraperitoneal injection. Control: treated with PBS and vehicle (DMSO 1% and Pluronic F-127 0.2%); LMM6: treated with LMM6 compound (5 mg/kg) and FLC: treated with fluconazole (5 mg/kg). (A) Fungal burden in the kidneys of the mice. (B) Fungal burden in the spleen of the mice. Colony Forming Units (log10 CFU) per gram of organ. Each data point represents the mean ± standard deviation (error bars). * Values of p < 0.05 were considered statistically significant. (C) Surfaces of the kidneys of untreated mice (control) covered with Candida lesions, while the kidneys of the LMM6-treated or FLC mice with healthy appearance. (D) Kidney histological section stained with hematoxylin eosin and Grocott-Gomori. The bar in the images corresponds to 50 μm. White arrows indicate the presence of fungus in the tissue that were easily spotted in the kidneys of control group (a and a’), whereas few were detected in the kidneys of LMM6-treated mice (b and b’). No fungus was observed on the FLC (c and c’). Representative kidney histopathological sections from 5 mice per group.