Figure 2.

Embryo survival and fly viability at 18 °C, 25 °C and 29 °C of Drosophila melanogaster wild type strain Canton-S [Bloomington Stock ID 64349], temperature sensitive shibire strain shi^ts2 (♀ shi^ts2/shi^ts2 and ♂ shi^ts2/Y) [Bloomington Stock ID 2248] and shibire Y-chromosome male rescue strain (♀ shi^ts2/shi^ts2 and ♂ shi^ts2/Dp(1:Y)shi⁺), generated from crossing shi^ts2 and Dp(1:Y)shi⁺ [Bloomington stock ID 4166]. (A) Embryos up to four hours old were collected and maintained at 18 °C, 25 °C and 29 °C for 48–72 h. The mean (±SD) percentage of hatched embryos from three replicates are shown, with 50 eggs per replicate. Significance was assessed using a two-way ANOVA followed by a Tukey post hoc test. The same letter above each bar indicates no significant difference (p > 0.05). High embryo mortality rates are observed in the shi^ts2 strain at 29 °C. (B) All strains tested are viable at 25 °C. (C) At 29 °C no survivorship is observed for the shi^ts2 strain, unless males carry the Y-chromosome rescue. Data is displayed as a percentage with the total number of flies indicated below each bar. Standard deviations from six to eight replicates are shown (Supplementary Methods S1).