Figure 3.

Embryo survival and fly viability at 22 °C, 25 °C and 29 °C of Drosophila melanogaster control strain w¹¹¹⁸ [Bloomington Stock ID 51629], temperature sensitive RpII215^ts (♀ RpII215^ts/RpII215^ts and ♂ RpII215^ts/Y) [Bloomington Stock ID 34755] and their hybrid progeny (♀ RpII215^ts/RpII215⁺ and ♂ RpII215^ts/Y). (A) Embryos up to six hours old were collected and maintained at three temperatures for 48–72 h. The mean (±SD) percentage of hatched embryos from four replicate are shown, with 40 eggs per replicate. Significance was assessed using a two-way ANOVA followed by a Tukey post hoc test. The same letter above each bar indicates no significant difference (p > 0.05). Observed female and male fly viability at (B) 22 °C, (C) 25 °C and (D) 29 °C temperatures confirmed rearing at 29 °C is lethal for homozygous females and hemizygous males, but not heterozygous females. Data is displayed as a percentage with the total number of flies indicated below each bar. Standard deviations from six replicates are shown (Supplementary Methods S1).