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. 2021 Mar 1;10(3):272. doi: 10.3390/pathogens10030272

Figure 4.

Figure 4

Effect of non-cytotoxic dilutions of Listerine and chlorhexidine gluconate on infection by pseudotyped SARS-CoV-2 viruses. (A) Pseudotyped SARS-CoV-2 virus (100 μL) was incubated with or without non-cytotoxic dilutions of Listerine or CHG at 37 °C for 30 min and then added to HeLa-hACE2 cells. After 1–2 h incubation, an additional 400 μL of Dulbecco’s Modified Eagle’s Medium (DMEM) 10% fetal bovine serum (FBS) was added to the cells without washing off the viruses or mouth rinses. Infected cells were cultured in the presence of mouth rinses for 2 days before measuring luciferase activity. (B) Pseudotyped SARS-CoV-2 viruses were pre-incubated with diluted CHG at 37 °C for 30 min (left panel) or without 30 min preincubation (right panel). Treated viruses were added to HeLa-hACE2 cells and incubated for 1 h at 37 °C. Cells were washed to remove residual mouth rinse and cultured for 2 days before measuring luciferase activity. The significance of differences between mouth rinse-treated viruses and mocked-treated controls (0%) was compared; * p < 0.05. Data are means ±SD.