Figure 3.

Impairment of motor function and anxiety-like behaviors in A30P*A53T*α-Syn transgenic mice. (a) Comparison of spontaneous locomotor activity between 5-month-old A30P*A53T*α-Syn and non-Tg mice in the open field test. Total distance traveled, fast movements, average speed and vertical count were significantly decreased in A30P*A53T*α-Syn mice compared with non-Tg mice. In parallel, transgenic mice showed an increased resting time compared with non-Tg mice. (b) Representative locomotor activity tracking was obtained from both phenotypes. (c) No difference in motor asymmetry examined by the use of the left paw was detected. However, A30P*A53T*α-Syn mice showed a reduced vertical count in the cylinder test compared with non-Tg mice. (d) In the dark-light box test, A30P*A53T*α-Syn transgenic mice evoked an anxiety-like response compared with non-Tg mice, as shown by the shorter spent time in the lit box but the marginal effects in the latency period to entering in the light box or the number of entries. Data are the mean ± SEM, n = 10–15 mice/group. Student’s t-test, * p < 0.05, ** p < 0.01 compared with non-Tg mice. See also Supplemental Figure S2.