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. 2021 Mar 13;22(6):2939. doi: 10.3390/ijms22062939

Figure 4.

Figure 4

A30P*A53T*α-Syn overexpression in TH+ neurons alters DA neurotransmission in the nigrostriatal pathway. (a) Local veratridine infusion (depolarizing agent; 50 μM) significantly increased DA release in the caudate putamen (CPu) of both mouse phenotypes. However, this effect was significant smaller in A30P*A53T*α-Syn mice than in non-Tg mice. (b) Direct application of amphetamine (DA releaser and DAT inhibitor; 1 and 10 μM) by reverse dialysis induced increases in DA release in the CPu, with the effect being significantly more pronounced in A30P*A53T*α-Syn mice than in non-Tg mice. (c) However, local nomifensine infusion (DAT inhibitor; 1 and 10 μM) dose-dependently increased the extracellular DA concentration in CPu, with this effect comparable being in both phenotypes. (d) Local activation of DA D2 receptors with quinpirole (DA D2 agonist, 10 μM) similarly decreased striatal DA release in A30P*A53T*α-Syn and non-Tg mice. Data are expressed as the mean ± SEM, n = 4–6 mice/group as indicated in the parenthesis. Two-way ANOVA and Tukey’s multiple comparisons test, ** p < 0.01, *** p < 0.001 compared with non-Tg mice.