Table 4.

(a–e). Multiple comparison test of LSmeans of nutrient concentrations (mg kg⁻¹) in partitioned plant tissues at various stages comparing treatment effects with control.

(a)
Location	Component and Stages	Control		T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1		T3R2	T2R1 & T3R1
B	Leaf
	V6	3.3(0.7) †,‡		3.3 ‡	3.0 ‡	3.5 ‡	4.0 ‡	3.0 ‡	4.8 ‡		5.0 + ‡	4.5 ‡
	V13	6.8(0.8) ‡		7.5 §	8.3 +§	6.5 ‡	7.3 ‡	8.2 +§	6.0 ‡		7.0 ‡	7.0 ‡
	V17	8.8(1.7) ‡		9.7	10.3	14.0 **§	14.7 ***§	14.5 **§	8.8 ‡		9.2 ‡	14.0 **§
	R2	7.3(0.8) ‡		7.2	8.2	8.7 +	8.0	8.3	8.8 +§		12.2 ***§	9.0 *§
	R6	4.8(0.6) ‡		4.5	4.8	6.0 *	4.5	5.0	5.0		5.7	4.8
	Stalk
	V6	5.5(0.4) ‡		4.8 ‡	5.5 ‡	5.0 ‡	5.8 ‡	5.3 ‡	5.0 ‡		4.5 +‡	5.0 ‡
	V13	5.2(0.9) ‡		6.0 §	5.7 §	4.8 ‡	5.3 ‡	7.0 +§	4.8 ‡		5.7 ‡	4.2 ‡
	V17	3.8(0.8) ‡		5.0	5.8 *	5.2 §	6.7 **§	6.5 **§	4.3 ‡		4.7 ‡	5.8 *§
	R2	4.2(0.6) ‡		5.7 *	4.8	5.5 *	4.8	6.0 **	5.3 +§		5.8 **§	4.8 §
	R6	1.0(1.0) ‡		1.0	1.2	2.3	1.0	1.2	1.0		2.5	1.2
	Reproductive
	R2	6.0(2.2) ‡		5.5	6.5	6.2	6.0	5.3	6.3 §		7.8 §	10.3 +§
	R6	2.3(1.0) ‡		6.0 ***	2.2	2.7	2.7	2.8	3.0		3.3	2.5
(b)
Location	Component and Stages	Control		T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1		T3R2	T2R1 & T3R1
Mn	Leaf
	V7	76.8(6.4) ‡		72.0 ‡	76.3 ‡	75.3 ‡	68.3 ‡	69.3 ‡	67.8 ‡		74.3 ‡	83.8 ‡
	V15	65.7(13.8) ‡		118.5 ***§	141.3 ***§	79.3 ‡	68.8 ‡	101.7 **§	69.0 ‡		78.3 ‡	77.2 ‡
	VT	83.5(10.4) ‡		102.7 +	123.8 ***	108.7 *§,¶	118.2 **§	129.7 ***§	79.8 ‡		76.8 ‡	115.7 **§
	R3	104.7(11.8) ‡		116.3	174.2 ***	106.7	133.7 *	136.5 **	132.7 *§		161.2 ***§	153.2 ***§
	R6	150.0(9.9) ‡		163.7	203.8 ***	159.7	182.2 **	184.2 ***	177.3 **		206.8 ***	195.2 ***
	Stalk
	V7	75.5(6.5) ‡		79.8 ‡	90.0 ‡	85.0 ‡	77.3 ‡	77.0 ‡	82.3 ‡		72.5 ‡	84.0 ‡
	V15	77.7(7.5) ‡		86.7 §	101.0 **§	68.7 ‡	76.2 ‡	83.3 §	75.8 ‡		70.2 ‡	72.3 ‡
	VT	87.5(6.6) ‡		81.3	80.2	84.7 §,¶	87.8 §	87.3 §	97.3 ‡		76.7 ‡	86.5 §
	R3	78.2(7.8) ‡		86.0	93.5+	80.3	104.3 **	99.7 **	94.7 *§		120.8 ***§	101.5 **§
	R6	40.8(6.0) ‡		34.3	43.7	27.3 *	42.8	49.2	43.2		46.7	43.3
	Reproductive
	VT	79.3(13.6) ‡		88.8	83.3	114.0 *§,¶	89.5 §	94.3 §	86.0 ‡		87.7 ‡	89.0§
	R3	23.3(5.4) ‡		21.3	25.7	25.0	24.0	26.7	31.5 §		36.5 *§	26.2 §
	R6	15.2(5.2) ‡		18.7	17.7	15.8	14.0	18.0	22.8		22.0	21.3
	Grain
	R3	15.9(1.8) ‡		13.7	12.0 *	12.3 *	13.9	13.7	15.3 §		13.6 §	14.1 §
	R6	5.8(0.3) ‡		6.0	6.1	5.9	6.1	5.7	5.9		6.3 +	5.8
(c)
Location	Component and Stages	Control		T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1		T3R2	T2R1 & T3R1
Zn only	Leaf
	V6	26.3(0.9 )‡		27.5 ‡	26.5 ‡	25.0 ‡	25.8 ‡	25.3 ‡	26.8 ‡		27.8 ‡	27.8 ‡
	V14	24.7(5.9) ‡		71.8 ***§	101.0 ***§	22.7 ‡	21.7 ‡	62.0 ***§,#	24.2 ‡		22.0 ‡	22.5 ‡
	V17	27.3(9.5) ‡		58.2 **	85.5 ***	78.2 ***§	108.8 ***§	91.3 ***§,#	27.3 ‡		28.5 ‡	73.3 ***§
	R2	29.2(22.0) ‡		49.0	80.0 *	54.5	74.2 *	123.2 ***	63.0 §		84.0 *§	88.5 **§
	R6	34.2(7.6) ‡		47.8 +	73.5 ***	57.8**	73.3 ***	68.0 ***	50.7 *		77.2 ***	71.2 ***
	Stalk
	V6	55.3(3.2) ‡		52.0 ‡	46.5 +‡	50.0 ‡	53.3 ‡	47.5 ‡	60.0 ‡		53.3 ‡	55.5 ‡
	V14	26.8(5.0) ‡		39.3 *§	73.3 ***§	37.5 *‡	32.3 ‡	42.7 **§,#	29.5 ‡		30.5 ‡	35. 3 ‡
	V17	23.7(3.0) ‡		27.0	45.8 ***	36.2 ***§	55.5 ***§	44.0 ***§,#	24.7 ‡		24.7 ‡	35.2 ***§
	R2	19.0(3.5) ‡		26.0 +	31.5 ***	27.3 *	41.0 ***	36.0 ***	27.5 *§		44.0 ***§	41.0 ***§
	R6	27.0(6.3) ‡		25.8	28.5	29.8	37.8 +	30.3	31.3		41.2 *	37.0
	Reproductive
	R2	26.5(2.8) ‡		28.7	30.3	29.3	34.7 **	32.8 *	38.2 ***§		45.3 ***§	41.2 ***§
	R6	34.7(5.9) ‡		34.3	43.0	30.0	35.2	34.2	30.3		39.8	39.7
	Grain
	R2	47.5(2.3) ‡		48.8	52.1 +	50.9	49.9	52.6 *	55.2 **§		53.9 **§	58.0 ***§
	R6	19.3(0.6) ‡		19.8	20.3	18.6	19.4	19.7	20.5+		19.9	20.1
(d)
Location	Component and Stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T4R1	T4R2	T1R1 & T4R1	T3R1 & T4R1
Fe/Zn (Zn Values)	Leaf
	V6	25.8(0.9) ‡	25.5 ‡	25.5 ‡	26.0 ‡	28.5 *‡	28.0 +‡	25.0 ‡	26.5 ‡	26.0 ‡	27.0 ‡	24.8 ‡	24.8 ‡
	V14	22.3(13.6) ‡	74.8 **§	53.8 §	51.5 ‡	40.0 ‡	68.5 *§	30.5 ‡	24.0 ‡	29.0 ‡	51.0 ‡	50.8 §	27.3 ‡
	V17	31.3(6.1) ‡	63.5 ***	65.0 ***	56.5 **§	80.0 ***§	80.3 ***§	26.3 ‡	27.8 ‡	24.8 ‡	25.3 ‡	49.3 *	25.0 ‡
	R2	34.5(7.8) ‡	55.5 +	86.0 ***	49.5	73.8 **	69.3 **	40.0 §	66.8 **§	31.3 ‡	34.0 ‡	71.0 **	55.5 +§
	R6	29.5(2.7) ‡	32.3	50.5 ***	32.0	43.3 ***	44.8 ***	30.3	36.8 +	30.3 §	39.3 *§	40.8 **§	37.5 *§
	Stalk
	V6	51.0(3.2) ‡	44.8 ‡	50.3 ‡	46.0 ‡	62.3 *‡	52.5 ‡	42.4 +‡	54.0 ‡	49.0 ‡	52.8 ‡	44.8 ‡	54.3 ‡
	V14	35.3(4.4) ‡	52.3 **§	51.3 *§	50.8 *‡	37.3 ‡	44.0 §	32.5 ‡	29.5 ‡	40.0 ‡	45.8 +‡	48.3 *§	48.5 *‡
	V17	26.3(3.3) ‡	29.0	38.8 **	31.3 §	31.0 §	31.0 §	26.3 ‡	23.0 ‡	23.8 ‡	28.3 ‡	34.3 +	21.5 ‡
	R2	21.0(2.8) ‡	18.8	22.5	19.8	24.8	23.8	23.0 §	23.5 §	18.0 ‡	17.8 ‡	22.5	25.3 §
	R6	18.8(2.8) ‡	15.0	15.8	16.5	12.0 +	15.5	15.8	10.8	12.5 §	16.8 §	12.8 §	15.5 §
	Reproductive
	R2	29.5(2.8) ‡	31.3	31.8	37.0 +	32.0	35.3	36.8 +§	37.3 +§	36.8 +‡	31.0 ‡	34.0	35.3 §
	R6	27.8(2.8) ‡	28.0	32.3	28.3	31.8	34.8 +	28.3	31.3	26.5 §	32.3 §	30.3 §	29.8 §
	Grain
	R2	48.4(5.9) ‡	65.2+	56.1	55.1	61.9	68.7 *	77.2 **§	66.8 *§	52.3 ‡	58.2 ‡	63.5 +	66.2 *§
	R6	19.4(1.0) ‡	18.5	19.3	17.8	19.5	20.8	18.5	18.9	18.3 §	18.4 §	18.7 §	19.5 §
(e)
Location	Component and Stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T4R1	T4R2	T1R1 & T4R1	T3R1 & T4R1
Fe/Zn (Fe Values)	Leaf
	V6	305.0(18.9 )‡	281.5 ‡	284.3 ‡	265.3 ‡	321.8 ‡	306.5 ‡	275.8 ‡	316.8 ‡	286.5 ‡	282.0 ‡	275.8 ‡	282.0 ‡
	V14	133.3(17.0) ‡	170.0 §	142.0 §	159.8 ‡	152.3 ‡	171.5 §	128.5 ‡	138.5 ‡	162.0 ‡	139.8 ‡	143.3 §	119.5 ‡
	V17	143.0(13.5) ‡	163.0	163.0	153.8 §	183.3 *§	184.0 *§	131.8 ‡	120.3 ‡	132.3 ‡	116.9 ‡	137.8	124.3 ‡
	R2	330.3(38.2) ‡	277.8	319.3	253.0	350.3	248.5	189.8 **§	264.3 §	274.5 ‡	213.3 *‡	197.0 *	286.8 §
	R6	98.5(7.5) ‡	94.3	107.3	94.3	103.3	99.3	82.5	98.8	84.8 §	94.5 §	89.0 §	103.5 §
	Stalk
	V6	177.5(25.9) ‡	187.3 ‡	159.5 ‡	157.0 ‡	166.8 ‡	194.5 ‡	152.3 ‡	211.3 ‡	176.0 ‡	160.3 ‡	194.3 ‡	158.8 ‡
	V14	33.5(5.3) ‡	34.5 §	38.0 §	35.0 ‡	42.5 ‡	40.3 §	32.0 ‡	39.8 ‡	40.5 ‡	44.5 ‡	34.5 §	37.5 ‡
	V17	37.3(10.8) ‡	35.0	32.3	32.3 §	50.3 §	34.5 §	40.8 ‡	59.3 ‡	40.5 ‡	38 ‡	32.8	38.5 ‡
	R2	86.8(12.7) ‡	50.0 *	52.3 +	44.0 *	74.8	72.3	69.0 §	56.8 §	58.0 ‡	53.8 +‡	63.0	46.0 *§
	R6	23.5(5.5) ‡	29.5	28.3	24.8	28.0	33.3	33.5	40.5 *	36 §	26.8 §	30 §	25.8 §
	Reproductive
	R2	73.3(15.7) ‡	110.8	76.5	61.5	58.0	74.3	69.8 §	52.8 §	61.3 ‡	51.0 ‡	64.5	80.3 §
	R6	33.8(6.8) ‡	30.0	27.8	31.0	33.5	42.8	30.5	34.8	23.8 §	26.3 §	31.8 §	29.8 §
	Grain
	R2	33.5(5.0) ‡	42.1	38.3	36.0	47.5 *	45.0	49.5 *§	42.8 §	36.0 ‡	44.9 ‡	42.3	43.1 §
	R6	19.0(1.1) ‡	17.6	21.6	17.8	19.2	19.4	18.3	18.0	17.0 §	16.9 §	17.6 §	18.7 §

† Least square mean plant nutrient concentration (mg kg⁻¹) followed by (SE for all values in the same row) and significant F test: Not Significant >0.10; + >0.05; * >0.01; **> 0.001; ***< 0.001. ‡ No foliar treatment had been applied at this stage. § First sampling following foliar treatment. ¶ First sampling following foliar treatment for plots with significant increase on grain yield. # First sampling following foliar treatment for plots with significant decrease on grain yield.